Localization of Myosin 1b to Actin Protrusions Requires Phosphoinositide Binding*
نویسندگان
چکیده
منابع مشابه
Localization of myosin 1b to actin protrusions requires phosphoinositide binding.
Myosin 1b (Myo1b), a class I myosin, is a widely expressed, single-headed, actin-associated molecular motor. Transient kinetic and single-molecule studies indicate that it is kinetically slow and responds to tension. Localization and subcellular fractionation studies indicate that Myo1b associates with the plasma membrane and certain subcellular organelles such as endosomes and lysosomes. Wheth...
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Myosin IIIA (MYO3A) targets actin protrusion tips using a motility mechanism dependent on both motor and tail actin-binding activity [1]. We show that myosin IIIB (MYO3B) lacks tail actin-binding activity and is unable to target COS7 cell filopodia tips, yet is somehow able to target stereocilia tips. Strikingly, when MYO3B is coexpressed with espin-1 (ESPN1), a MYO3A cargo protein endogenously...
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The studies of Szent-Gyorgyi (1) have clearly established the formation of actomyosin from F-actin and myosin A. SzentGyorgyi (l), Portzehl, Schramm, and Weber (2), Jaisle (3), Snellman and Gelotte (4), and Spicer and Gergely (5) have suggested from their viscometric and centrifugal studies that the maximal amount of myosin A which can be bound by F-actin is in the ratio of 2 to 4 : 1, by weigh...
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Nonmuscle myosin II plays fundamental roles in cell body translocation during migration and is typically depleted or absent from actin-based cell protrusions such as lamellipodia, but the mechanisms preventing myosin II assembly in such structures have not been identified [1-3]. In Dictyostelium discoideum, myosin II filament assembly is controlled primarily through myosin heavy chain (MHC) pho...
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Nonmuscle myosin II plays fundamental roles in cell body translocation during migration and is typically depleted or absent from actin-based cell protrusions such as lamellipodia, but the mechanisms preventing myosin II assembly in such structures have not been identified [1], [2] and [3]. In Dictyostelium discoideum, myosin II filament assembly is controlled primarily through myosin heavy chai...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2010
ISSN: 0021-9258
DOI: 10.1074/jbc.m109.087270